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Contagem de reticulócitos de cães saudáveis ou anêmicos pela citometria de fluxo Arq. Bras. Med. Vet. Zootec.
Pereira,P.M.; Seki,M.C.; Palma,P.V.B.; Morais,F.R.; Santana,A.E.; Pereira,G.T..
Compararam-se os resultados da contagem de reticulócitos pela microscopia de luz e pelo método da citometria de fluxo em 25 cães saudáveis (controle), 60 cães com anemia regenerativa e 40 com anemia arregenerativa. Houve diferença nas contagens absolutas obtidas pela microscopia de luz e pela citometria de fluxo nos três grupos estudados. A contagem de reticulócitos foi mais alta pela citometria de fluxo que a contagem pela microscopia de luz, mostrando ser um método mais sensível, simples e seguro para a quantificação de reticulócitos.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cão; Citometria de fluxo; Anemia; Reticulócitos.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000100010
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Immunological effects of donor lymphocyte infusion in patients with chronic myelogenous leukemia relapsing after bone marrow transplantation BJMBR
Castro,F.A.; Palma,P.V.B.; Morais,F.R.; Voltarelli,J.C..
Allogeneic bone marrow transplantation (alloBMT) is the only curative therapy for chronic myelogenous leukemia (CML). This success is explained by the delivery of high doses of antineoplastic agents followed by the rescue of marrow function and the induction of graft-versus-leukemia reaction mediated by allogeneic lymphocytes against host tumor cells. This reaction can also be induced by donor lymphocyte infusion (DLI) producing remission in most patients with CML who relapse after alloBMT. The immunological mechanisms involved in DLI therapy are poorly understood. We studied five CML patients in the chronic phase, who received DLI after relapsing from an HLA-identical BMT. Using flow cytometry we evaluated cellular activation and apoptosis, NK...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Chronic myelogenous leukemia; Donor lymphocyte infusion; Immune system; Bone marrow transplantation.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000200006
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Mesenchymal stem cells from patients with chronic myeloid leukemia do not express BCR-ABL and have absence of chimerism after allogeneic bone marrow transplant BJMBR
Carrara,R.C.V.; Orellana,M.D.; Fontes,A.M.; Palma,P.V.B.; Kashima,S.; Mendes,M.R.; Coutinho,M.A.; Voltarelli,J.C.; Covas,D.T..
Bone marrow is a heterogeneous cell population which includes hematopoietic and mesenchymal progenitor cells. Dysregulated hematopoiesis occurs in chronic myelogenous leukemia (CML), being caused at least in part by abnormalities in the hematopoietic progenitors. However, the role of mesenchymal stem cells (MSCs) in CML has not been well characterized. The objectives of the present study were to observe the biological characteristics of MSCs from CML patients and to determine if MSCs originate in part from donors in CML patients after bone marrow transplantation (BMT). We analyzed MSCs from 5 untreated patients and from 3 CML patients after sex-mismatched allogeneic BMT. Flow cytometry analysis revealed the typical MSC phenotype and in vitro assays showed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mesenchymal stem cells; Chimerism; Allogeneic hematopoietic stem cell transplantation; Chronic myeloid leukemia; Bcr-abl gene.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000100008
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Overexpression of hsa-miR-125b during osteoblastic differentiation does not influence levels of Runx2, osteopontin, and ALPL gene expression BJMBR
Pinto,M.T.; Nicolete,L.D.F.; Rodrigues,E.S.; Palma,P.V.B.; Orellana,M.D.; Kashima,S.; Covas,D.T..
Multipotent mesenchymal stromal cells (MSCs) were first isolated from bone marrow and then from various adult tissues including placenta, cord blood, deciduous teeth, and amniotic fluid. MSCs are defined or characterized by their ability to adhere to plastic, to express specific surface antigens, and to differentiate into osteogenic, chondrogenic, adipogenic, and myogenic lineages. Although the molecular mechanisms that control MSC proliferation and differentiation are not well understood, the involvement of microRNAs has been reported. In the present study, we investigated the role of miR-125b during osteoblastic differentiation in humans. We found that miR-125b increased during osteoblastic differentiation, as well as Runx2 and ALPL genes. To study...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Multipotent mesenchymal stromal cells; Osteoblastic differentiation; Hsa-miR-125b.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000800676
Registros recuperados: 4
Primeira ... 1 ... Última
 

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